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Production and Structural Dynamics of Microbial Rhodopsins


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Title: Production and Structural Dynamics of Microbial Rhodopsins
Authors: Wickstrand, Cecilia
E-mail: cecilia.wickstrand@gu.se
Issue Date: 22-May-2019
University: University of Gothenburg. Faculty of Science
Institution: Department of Chemistry and Molecular Biology ; Institutionen för kemi och molekylärbiologi
Parts of work: 1. Cecilia Wickstrand et al., “Production and purification of channelrhodopsins expressed in Pichia Pastoris”, Manuscript (2019)

2. Cecilia Wickstrand et al., “Bacteriorhodopsin: Would the real structural intermediates please stand up?”, Biochimica et Biophysica Acta 1850 (3): 536‐553 (2015),
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3. Nogly et al., “Lipidic cubic phase serial millisecond crystallography using synchrotron radiation”, Journal of the International Union of Crystallography 2 (2): 168‐ 176 (2015),
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4. Nango et al., “A three‐dimensional movie of structural changes in bacteriorhodopsin”, Science 354 (6319): 1552‐1557 (2016),
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5. Wickstrand et al., “A tool for visualizing protein motions in time‐resolved crystallography”, Manuscript (2019)

6. “Bacteriorhodopsin: Structural insights revealed using X‐Ray lasers and synchrotron radiation”, Annual Review of Biochemistry 88 (2019),
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Date of Defence: 2019-06-12
Disputation: Onsdagen den 12:e juni 2019 kl. 09:30, hörsal Ivan Ivarsson, Medicinaregatan 3
Degree: Doctor of Philosophy
Publication type: Doctoral thesis
Keywords: bacteriorhodopsin
channelrhodopsin
time-resolved serial femtosecond crystallography
Abstract: Rhodopsins are a family of membrane proteins that are found in a wide range of organisms and provide them with the ability to sense and react to light. When light is absorbed by these proteins, structural changes occur that initiate the pumping of protons, opening of a channel or signal transfer. The rhodopsins have different functions but they are structurally similar. This makes the protein family interesting from the perspective of structural biology, a field that aims to link function of pro... more
ISBN: 978‐91‐7833‐493‐3
URI: http://hdl.handle.net/2077/60173
Appears in Collections:Doctoral Theses from University of Gothenburg / Doktorsavhandlingar från Göteborgs universitet
Doctoral Theses / Doktorsavhandlingar Institutionen för kemi och molekylärbiologi

 

 

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