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Lipidic Cubic Phase Microcrystallization and its Application in Serial Crystallography


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Title: Lipidic Cubic Phase Microcrystallization and its Application in Serial Crystallography
Authors: Andersson, Rebecka
E-mail: Gusrebecan@gmail.com
Issue Date: 23-Oct-2020
University: University of Gothenburg. Faculty of Science
Institution: Department of Chemistry and Molecular Biology ; Institutionen för kemi och molekylärbiologi
Parts of work: Paper I. R. Andersson*, C. Safari*, P. Båth, R. Bosman, A. Shilova, P. Dahl, S. Gosh, A. Dunge, R. Kjeldsen-Jensen, J. Nan, R. L. Shoeman, M. Kloos, R. B. Doak, U. Mueller, R. Neutze & G. Brändén, (2019). “Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography”. Acta Cryst. D75, 937-946,
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Paper II. R. Andersson, C. Safari, R. Dods, E. Nango, R. Tanaka, A. Yamashita, T. Nakane, K. Tono, Y. Joti, P. Båth, E. Dunevall, R. Bosman, O. Nureki, S. Iwata, R. Neutze and G. Brändén. (2017). “Serial femtosecond crystallography structure of cytochrome c oxidase at room temperature”. Scientific Reports 7,
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Paper III. C. Safari, R. Andersson, S. Gosh, J. Johannesson, P. Båth, R. Bosman, P. Dahl, E. Nango, R. Tanaka, E. Dunevall, P. Börjesson, O. Uwangue, D. Zoric, G. Hammarin, M. Panman, E. Svensson, G. Ortolani, T. Tanaka, T. Tosha, H. Takeda, H. Naitow, T. Arima, A. Yamashita, M. Sugahara, T. Nakane, O. Nureki, S. Iwata, R. Neutze and G. Brändén. “Room-temperature structure of CO-bound ba3-type cytochrome c oxidase reveals mechanistic differences between A-type and B-type enzymes” Manuscript (2020)

Paper IV. P. Båth, P. Börjesson, R. Bosman, C. Wickstrand, R. Dods, T. B. Úlfarsdóttir, P. Dahl, M. J. García-Bonete, J. B. Linse, G. Ortolani, R. Andersson, C. Safari, E. Dunevall, S. Ghosh, E. Nango, R. Tanaka, T. Nakane, A. Yamashita, K. Tono, Y. Joti, T. Tanaka, S. Owada, T. Arima, O. Nureki, S. Iwata, G. Brändén and R. Neutze. “Lipidic cubic phase serial femtosecond crystallography structure of a photosyntecic reaction centre” Manuscript (2020)

Paper V. R. Bosman, G. Ortolani, S. Ghosh, T. B. Úlfarsdóttir, D. James, P. Börjesson, G. Hammarin, R. Andersson, C. Safari, T. Weinert, F. Dworkowski, T. Takashi, J. Standfuss, G. Brändén and R. Neutze, “Structural basis for the prolonged photocycle of Sensory Rhodopsin II revealed by serial millisecond crystallography” Manuscript (2020)
Date of Defence: 2020-11-25
Disputation: Onsdagen den 25 november 2020, kl. 13.00, Hörsal Arvid Carlsson, Academicum, Medicinaregatan 3.
Degree: Doctor of Philosophy
Publication type: Doctoral thesis
Keywords: Structural biology
Lipidic Cubic Phase
Cytochrome c oxidase
Serial Crystallography
Abstract: Every living organism contain a cell membrane which is an important cell structure with a vast variety of different functions such as cell signaling, transportation and energy production. One of the most important functions is to produce energy for the cell to thrive. In humans and other organisms, oxygen is used as the final electron acceptor to drive reactions that pump protons across the membrane to create an electrochemical proton gradient. This electrochemical proton gradient is then harves... more
ISBN: 978-91-8009-114-5
978-91-8009-115-2
URI: http://hdl.handle.net/2077/66804
Appears in Collections:Doctoral Theses from University of Gothenburg / Doktorsavhandlingar från Göteborgs universitet
Doctoral Theses / Doktorsavhandlingar Institutionen för kemi och molekylärbiologi

 

 

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