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Isotope labeling of proteins for spectroscopic studies


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Title: Isotope labeling of proteins for spectroscopic studies
Authors: Gustavsson, Emil
E-mail: emil.gustavsson@cmb.gu.se
Issue Date: 15-Nov-2018
University: University of Gothenburg. Faculty of Science
Institution: Department of Chemistry and Molecular Biology ; Institutionen för kemi och molekylärbiologi
Parts of work: E. Gustavsson*, L. Isaksson*, C. Persson, U. Brath, M. Mayzel, J. Ihalainen, G. Karlsson, V. Orekhov, and S. Westenhoff, “Solution NMR spectroscopy reveals that the PHY-tongue region of a bacteriophytochrome undergoes a soft-rigid transition when the protein is photoactivated” Manuscript, (2018)

E. Gustavsson*, L. Isaksson*, C. Persson, U. Brath, G. Karlsson, V. Orekhov, and S. Westenhoff, “The knot region of a bacterial phytochrome transduces the photosignal towards the output domains” Manuscript, (2018)

J. A. Ihalainen, E. Gustavsson*, L. Schröder*, S. Donnini, H. Lehtivuori, L. Isaksson, C. Thöing, V. Modi, O. Berntsson, B. Stucki-Buchli, A. Liukkonen, H. Häkkänen, E. Kalenius, S. Westenhoff, and T. Kottke, “Chromophore- Protein Interplay During the Phytochrome Photocycle Revealed by Step-Scan FTIR Spectroscopy” Journal of American Chemical Society, Sep. 2018.
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S. Peuker, H. Andersson, E. Gustavsson, K. S. Maiti, R. Kania, A. Karim, S. Niebling, A. Pedersen, M. Erdelyi, and S. Westenhoff, “Efficient Isotope Editing of Proteins for Site-Directed Vibrational Spectroscopy” Journal of American Chemical Society, vol. 138, no. 7, pp. 2312–2318, Feb. 2016.
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G. Kovacsova, E. Gustavsson, J. Wang, M. Kreir, S. Peuker, and S. Westenhoff, “Cell-free expression of a functional pore-only sodium channel” Protein Expression and Purification, vol. 111, pp. 42–47, Jul. 2015.
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Date of Defence: 2018-12-07
Disputation: Fredagen den 7 december 2018 kl.09.00, Hörsal Ragnar Sandberg, Medicinaregatan 7A
Degree: Doctor of Philosophy
Publication type: Doctoral thesis
Keywords: Isotope labeling
Proteins
Cell-free protein expression
NMR
IR
Infrared
Abstract: Protein structural dynamics have been closely tied to the molecular mechanisms controlling for example enzyme activities. In my work, I have mainly studied the bacteriophytochrome from Deinococcus radiodurans. Phytochromes are photoreceptors that monitor the level, intensity, duration and color of environmental light to regulate numerous fundamental photomorphogenic processes in plants, fungi, and bacteria. In order to monitor the dynamic nature of such proteins, they are preferably investigated... more
ISBN: 978-91-7833-080-5
978-91-7833-079-9
URI: http://hdl.handle.net/2077/57807
Appears in Collections:Doctoral Theses from University of Gothenburg / Doktorsavhandlingar från Göteborgs universitet
Doctoral Theses / Doktorsavhandlingar Institutionen för kemi och molekylärbiologi

 

 

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