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Multi-photon microscopy enhanced: Exploring annular beams and gold nano-particles for improved imaging


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Title: Multi-photon microscopy enhanced: Exploring annular beams and gold nano-particles for improved imaging
Authors: Borglin, Johan
E-mail: johan.t.borglin@gmail.com
Issue Date: 9-May-2016
University: Göteborgs universitet. Naturvetenskapliga fakulteten
Institution: Department of Physics ; Institutionen för fysik
Parts of work: J. Borglin et al. Improving multiphoton microscopy using annular beam shaping, focusing on imaging of human skin. Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482K (28 February 2014);
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J. Borglin et al. Insights on proximity effect and multiphoton induced luminescence from gold nanospheres in far field optical microscopy. Appl. Phys. Lett. 107, 234101 (2015)
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J. Borglin et al. Functionalized gold nanoparticles as switchable contrast medium in multiphoton microscopy.

J Borglin et al. Exploring annular beam shaping to improve imaging depth for multiphoton microscopy in turbid medium.
Date of Defence: 2016-05-27
Disputation: Fredagen den 27 Maj 2016, kl. 10. Hörsal KE, Kemihuset, Kemigården 10.
Degree: Doctor of Philosophy
Publication type: Doctoral thesis
Keywords: Multiphoton microscopy
Beam shaping
Gold nanoparticles
Abstract: Laser scanning multiphoton microscopy (MPM) has emerged as a powerful tool for non-invasive three-dimensional imaging of biological tissue. The localized focal region enables confocality without the use of a physical pinhole while providing less photobleaching and photodamage compared to confocal laser scanning microscopy. As imaging depth increases, the capabilities of MPM becomes significantly limited by overwhelming background fluorescence and decreased contrast, particularly within highly li... more
ISBN: 978-91-628-9786-4
URI: http://hdl.handle.net/2077/42385
Appears in Collections:Doctoral Theses from University of Gothenburg / Doktorsavhandlingar från Göteborgs universitet
Doctoral Theses / Doktorsavhandlingar Institutionen för fysik

 

 

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