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Measurements of the DNA double-strand break response

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Title: Measurements of the DNA double-strand break response
Authors: Muslimovic, Aida
Issue Date: 25-Sep-2012
University: University of Gothenburg. Sahlgrenska Academy
Institution: Institute of Biomedicine. Department of Clinical Chemistry and Transfusion Medicine
Parts of work: I. Numerical analysis of etoposide induced DNA breaks. Muslimović A, Nyström S, Gao Y, Hammarsten O.

II. An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells. Muslimovic A, Ismail IH, Gao Y, Hammarsten O.

III. Calibrators for clinical measurements of phosphorylated H2AX in patient cells by flow cytometry. Muslimovic A, Johansson P, Ruetschi U, Hammarsten O. Submitted manuscript.

IV. In-solution staining and arraying method for the immunofluorescence detection of γH2AX foci optimized for clinical applications. Johansson P, Muslimovic A, Hultborn R, Fernström E, Hammarsten O.

Date of Defence: 2012-10-19
Disputation: Fredagen den 19 oktober 2012, kl. 13.00, Hörsal Arvid Carlsson, Academicum, Medicinaregatan 3, Göteborg
Degree: Doctor of Philosophy (Medicine)
Publication type: Doctoral thesis
Keywords: DSB response
cancer patients
H2AX phosphorylation
Abstract: Radiotherapy and some chemotherapeutic drugs kill cancer cells by induction of the extremely toxic DNA double-strand breaks (DSBs). Measurements of the DSB response in patients during therapy could allow personalized dosing to improve tumor response and minimize side effects. DSBs induce a strong cellular response via phosphorylation of H2AX, P-H2AX and the formation of foci. P-H2AX can be measured by flow cytometry or counted in separate cell nuclei by immunofluorescence microscopy. The o... more
ISBN: 978-91-628-8504-5
Appears in Collections:Doctoral Theses from Sahlgrenska Academy
Doctoral Theses from University of Gothenburg / Doktorsavhandlingar från Göteborgs universitet
Doctoral Theses / Doktorsavhandlingar Institutionen för biomedicin



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