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Measurements of the DNA double-strand break response


Please use this identifier to cite or link to this item: http://hdl.handle.net/2077/30410

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Title: Measurements of the DNA double-strand break response
Authors: Muslimovic, Aida
E-mail: aida.muslimovic@clinchem.gu.se
Issue Date: 25-Sep-2012
University: University of Gothenburg. Sahlgrenska Academy
Institution: Institute of Biomedicine. Department of Clinical Chemistry and Transfusion Medicine
Parts of work: I. Numerical analysis of etoposide induced DNA breaks. Muslimović A, Nyström S, Gao Y, Hammarsten O.
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II. An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells. Muslimovic A, Ismail IH, Gao Y, Hammarsten O.
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III. Calibrators for clinical measurements of phosphorylated H2AX in patient cells by flow cytometry. Muslimovic A, Johansson P, Ruetschi U, Hammarsten O. Submitted manuscript.

IV. In-solution staining and arraying method for the immunofluorescence detection of γH2AX foci optimized for clinical applications. Johansson P, Muslimovic A, Hultborn R, Fernström E, Hammarsten O.
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Date of Defence: 2012-10-19
Disputation: Fredagen den 19 oktober 2012, kl. 13.00, Hörsal Arvid Carlsson, Academicum, Medicinaregatan 3, Göteborg
Degree: Doctor of Philosophy (Medicine)
Publication type: Doctoral thesis
Keywords: DSB response
cancer patients
H2AX phosphorylation
Abstract: Radiotherapy and some chemotherapeutic drugs kill cancer cells by induction of the extremely toxic DNA double-strand breaks (DSBs). Measurements of the DSB response in patients during therapy could allow personalized dosing to improve tumor response and minimize side effects. DSBs induce a strong cellular response via phosphorylation of H2AX, P-H2AX and the formation of foci. P-H2AX can be measured by flow cytometry or counted in separate cell nuclei by immunofluorescence microscopy. The o... more
ISBN: 978-91-628-8504-5
URI: http://hdl.handle.net/2077/30410
Appears in Collections:Doctoral Theses from Sahlgrenska Academy
Doctoral Theses from University of Gothenburg / Doktorsavhandlingar från Göteborgs universitet
Doctoral Theses / Doktorsavhandlingar Institutionen för biomedicin

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