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Structural Insights at Sub-Ångstrom, Medium and Low Resolution: Crystallization of Trypsin, Bacterioferritin, Photosynthetic Reaction Center, and Photosynthetic Core Complex


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Title: Structural Insights at Sub-Ångstrom, Medium and Low Resolution: Crystallization of Trypsin, Bacterioferritin, Photosynthetic Reaction Center, and Photosynthetic Core Complex
Authors: Yuan Wahlgren, Weixiao
E-mail: weixiao.yuanwahlgren@chem.gu.se
Issue Date: 28-Sep-2012
University: University of Gothenburg. Faculty of Science
Institution: Department of Chemistry ; Institutionen för kemi
Parts of work: I. Wahlgren WY, Pal G, Kardos J, Porrogi P, Szenthe B, Patthy A, Graf L, Katona G. (2011) The Catalytic Aspartate Is Protonated in the Michaelis Complex Formed between Trypsin and an in Vitro Evolved Substrate-like Inhibitor A REFINED MECHANISM OF SERINE PROTEASE ACTION. J. Biol. Chem. 286 (5), 3587-3596.
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II. Wöhri AB, Wahlgren WY, Malmerberg E, Johansson LC, Neutze R, Katona G. (2009) Lipidic Sponge Phase Crystal Structure of a Photosynthetic Reaction Center Reveals Lipids on the Protein Surface. Biochemistry 48 (41), 9831-9838.
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III. Wöhri AB, Johansson LC, Wadsten-Hindrichsen P, Wahlgren WY, Fischer G, Horsefield R, Katona G, Nyblom M, Oberg F, Young G, Cogdell RJ, Fraser NJ, Engström S, Neutze R. (2008) A lipidic-sponge phase screen for membrane protein crystallization. Structure 16 (7), 1003-1009.
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IV. Wahlgren WY, Omran H, von Stetten D, Royand A, van der Post S, Katona G. Structural characterization of bacterioferritin from Blastochloris viridis. Accepted for publication at PLoS ONE.
Date of Defence: 2012-10-19
Disputation: Fredagen den 19 oktober 2012, kl. 10.00, sal Karl Kylberg (K2320), Medicinaregatan 7B
Degree: Doctor of Philosophy
Publication type: Doctoral thesis
Keywords: protein crystallization, cubic phase, sponge phase, bicelle, trypsin, bacterioferritin, reaction center, core complex, photosynthesis
Abstract: The catalytic action of serine proteases depends on the interplay of a nucleophile, a general base and a general acid. The catalytic triad is composed of serine, histidine and aspartate residues. The serine acts as a nucleophile while the histidine plays a dual role as the general base or acid at different steps of the reaction. However, the role of aspartate is unclear. I recovered an ultrahigh resolution (0.93 Å) X-ray structure of a complex formed between trypsin and a canonical inhibitor. A... more
ISBN: 978-91-628-8539-7
URI: http://hdl.handle.net/2077/30181
Appears in Collections:Doctoral Theses from University of Gothenburg / Doktorsavhandlingar från Göteborgs universitet
Doctoral Theses / Doktorsavhandlingar Institutionen för kemi och molekylärbiologi

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