The Effects of Temperature on Gut Blood Flow and Gut Motility in Fish
Abstract
In order for a fish to grow and thrive, the gut must function efficiently. This is achieved through a range of processes, including controlled patterns of gut motility and modifications in gut blood flow. How these processes are affected by long and short-term changes in ambient temperature was the main focus of this thesis.
After acclimation to a higher temperature, the interdigestive motility of rainbow trout was higher. This indicates an increased demand for movements involved in the housekeeping functions of the gut. Temperature acclimation did not affect the postprandial response, in neither gut blood flow nor gut motility. These results indicate that thermal compensation processes work to neutralize acute thermal effects, so that these two functions are maintained at a certain rate. Also, in vitro preparations of isolated intestine showed signs of thermal compensation processes, as the acute temperature effects on the response to the cholinergic agonist carbachol were abolished after a temperature acclimation period.
Fish species studied responded very differently to an acute change in temperature. A relatively linear correlation with temperature was seen in, both gut blood flow and gut motility, in two species of sculpins (shorthorn and Arctic sculpin). The gut blood flow, in both green and white sturgeon, was unaffected by moderate fluctuations in water temperature during the interdigestive state. White sturgeon were also studied after feeding and a correlation between gut blood flow and temperature was observed, in a similar fashion as for the unfed sculpins.
Thermoregulatory behaviours observed in white sturgeon, show that moving between different temperatures, after a meal, will have a great influence on the volume of blood distributed to the gut. An increase in gut blood flow is probably an important factor explaining why it can be beneficial to migrate into warmer waters after feeding. However, if combining the presented data with data from previous studies, it shows that perfusion of the gut can also be a limiting factor when the environmental temperature changes. This is probably the reason why some fish species forage in warm waters and then move into colder areas when digesting the meal.
A leading hypothesis suggests that the temperature limitations for a fish are mainly set by a reduction in metabolic scope. However, experimental data has shown that temperature can have significant effects on both appetite and growth before metabolic scope is reduced. The findings in this thesis suggest that oxygen-limited thermal tolerance in the gut of fish could also be a significant variable in determining the temperature range that fish can tolerate.
Parts of work
I. Gräns A, Albertsson F, Axelsson M, Olsson C (2009) Postprandial changes in enteric electrical activity and gut blood flow in rainbow trout (Oncorhynchus mykiss) acclimated to different temperatures. Journal of Experimental Biology, 212, 2550-2557::doi::10.1242/jeb.030593 II. Gräns A, Seth H, Axelsson M, Sandblom E, Albertsson F, Wiklander K, Olsson C (2012) Effects of acute temperature changes on gut physiology in two species of sculpin from the west coast of Greenland (manuscript) III. Gräns A, Axelsson M, Olsson C, Höjesjö J, Pitsillides K, Kaufman R, Cech J (2009) A fully implantable multi-channel biotelemetry system for measurement of blood flow and temperature: a first evaluation in the green sturgeon. Hydrobiologia, 619, 11-25::doi::10.1007/s10750-008-9578-7 IV. Gräns A, Olsson C, Pitsillides K, Nelson H, Cech J, Axelsson M (2010) Effects of feeding on thermoregulatory behaviours and gut blood flow in white sturgeon (Acipenser transmontanus) using biotelemetry in combination with standard techniques. Journal of Experimental Biology, 213, 3198-3206::doi::10.1242/jeb.043570
Degree
Doctor of Philosophy
University
University of Gothenburg. Faculty of Science
Institution
Department of Zoology ; Zoologiska institutionen
Disputation
kl. 10.00 i Lyktan Konferenscentrum Wallenberg, Medicinaregatan 20A, Göteborg
Date of defence
2012-03-30
albin.grans@zool.gu.se
Date
2012-03-09Author
Gräns, Albin
Keywords
zoofysiologi
Publication type
Doctoral thesis
ISBN
ISBN 978-91-628-8446-8
Language
eng