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Towards quantitative single cell analysis using optical tweezers and microfluidics


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Title: Towards quantitative single cell analysis using optical tweezers and microfluidics
Authors: Eriksson, Emma
E-mail: emma.eriksson@physics.gu.se
Issue Date: 3-Apr-2009
University: University of Gothenburg. Faculty of Science.
Institution: Department of Physics ; Institutionen för fysik
Parts of work: I. Eriksson, E., Enger, J., Nordlander, B., Erjavec, N., Ramser, K., Goksör, M., Hohmann, S., Nyström, T. & Hanstorp, D. (2007). A microfluidic system in combination with optical tweezers for analyzing rapid and reversible cytological alterations in single cells upon environmental changes. Lab on a Chip, 7, 71-76.
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II. Eriksson, E., Scrimgeour, J., Granéli, A., Ramser, K., Wellander, R., Enger, J., Hanstorp, D. & Goksör, M. (2007). Optical manipulation and microfluidics for studies of single cell dynamics. Journal of Optics A: Pure and Applied Optics, 9, S113-S121.
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III. Eriksson, E., Scrimgeour, J., Enger, J. & Goksör, M. (2007). Holographic optical tweezers combined with a microfluidic device for exposing cells to fast environmental changes. Proceedings of SPIE, Vol. 6592, 65920P.
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IV. Eriksson, E., Keen, S., Leach, J., Goksör, M. & Padgett, M. J. (2007). The effect of external forces on discrete motion within holographic optical tweezers. Optics Express, 15(26), 18268-18274.
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V. Eriksson, E., Engström, D., Scrimgeour, J. & Goksör, M. (2009). Automated focusing of nuclei for time lapse experiments on single cells using holographic optical tweezers. Optics Express, 17(7), 5585-5594.
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VI. Eriksson, E., Sott, K., Lundqvist, F., Sveningsson, M., Scrimgeour, J., Hanstorp, D., Goksör, M. & Granéli, A. (2009). An experimental approach for quantitative studies of single cells in dynamically changing environments. Unpublished manuscript.

VII. Schaber, J., Angel Adrover, M., Eriksson, E., Pelet, S., Petelenz, E., Klein, D., Posas, F., Goksör, M., Peter, M., Hohmann, S. & Klipp E. (2009). Biophysical properties of Saccharomyces cerevisiae and their relation to HOG pathway activation. Unpublished manuscript.
Date of Defence: 2009-04-29
Disputation: Onsdagen den 29 april 2009, kl 9.00, sal Pascal, Matematiska vetenskaper, Hörsalsvägen 1
Degree: Doctor of Philosophy
Publication type: Doctoral thesis
Keywords: Optical tweezers
holographic optical tweezers
microfluidics
lab-on-a-chip
fluorescence microscopy
spatial light modulator
single cell analysis
quantitative systems biology
GFP
Saccharomyces cerevisiae
Abstract: Experiments on single cells have the potential to uncover information that would not be possible to obtain with traditional biological techniques, which only reflect the average behavior of a population of cells. In the averaging process, information regarding heterogeneity and cellular dynamics, that may give rise to a nondeterministic behavior at the population level, is lost. In this thesis I have demonstrated how optical tweezers, microfluidics and fluorescence microscopy can be combine... more
ISBN: 978-91-628-7751-4
URI: http://hdl.handle.net/2077/19485
Appears in Collections:Doctoral Theses / Doktorsavhandlingar Institutionen för fysik
Doctoral Theses from University of Gothenburg / Doktorsavhandlingar från Göteborgs universitet

 

 

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