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oai:gupea.ub.gu.se:2077/560752018-03-23T02:33:59Zcom_2077_29047com_2077_4716com_2077_10556col_2077_29054col_2077_10557

Edlund, Petra 2018-03-22T14:36:29Z 2018-03-22T14:36:29Z 2018-03-22 978-91-629-0472-2 http://hdl.handle.net/2077/56075 The key to life on earth is sunlight, which reaches the planet as an energy source. Nature has evolved different types of photoreceptor proteins to detect optimal light conditions for biochemical processes. A type of red light detecting photoreceptor proteins are called phytochromes and are present in plants, fungi and bacteria. A chromophore, converts the light signal into a structural change in the protein that alter its biochemical properties and thereby control developmental processes in the organism. A structural mechanism for signal transduction within the phytochrome protein is herein proposed. The aim of the work presented in this thesis has been to elucidate the structural changes in bacteriophytochromes upon photoactivation. This has been done by the use of X-ray crystallographic methods that can provide a near-atomic resolution of the dynamic events. Crystallization strategies were developed to experimentally obtain novel structural information on bacteriophytochromes from both conventional crystallography and by Serial Femtosecond Crystallography at X-ray Free electron lasers. The method enable time-resolved structural studies with an ultrafast timeresolution due to the X-ray lasers short pulses. Novel crystallization conditions for a bacteriophytochrome fragment yielded nearatomic resolution structures of both the wild type and a muted variant. The conditions could be modified for microcrystallization that provided microcrystals suitable for two different sample delivery systems at the world’s two most prominent X-ray lasers. The obtained resting state structures and a preliminary data set of the excited state paves the way for future time resolved investigation on the early structural events in photoactivation of phytochromes. Furthermore, the microcrystallization strategies might be applicable to other proteins and are thereby contributing to method development within the emerging field. The crystallographic structure of the mutated variant of the protein fragment supports IR-spectroscopy findings on the importance of the hydrogen bonding network around the chromophore. These results are in agreement with the excited state structural findings that waters might be of highest importance for the initial steps in the photoactivation of phytochromes. eng Petra Edlund, Heikki Takala, Janne.A Ihalainen, Sebastian Westenhoff. ”Structural Mechanism of Signaling in Bacteriophytochromes. “ Manuscript (2018) Petra Edlund,* Heikki Takala,* Elin Claesson,* Léocadie Henry, Robert Dods, Heli Lehtivuori, Matthijs Panman, Kanupriya Pande, Thomas White, Takanori Nakane, Oskar Berntsson, Emil Gustavsson, Petra Båth, Vaibhav Modi, Shatabdi Roy-Chowdhury, James Zook, Peter Berntsen, Suraj Pandey, Ishwor Poudyal, Jason Tenboer, Christopher Kupitz, Anton Barty, Petra Fromme, Jake D. Koralek, Tomoyuki Tanaka, John Spence, Mengning Liang, Mark S. Hunter, Sebastien Boutet, Eriko Nango, Keith Moffat, Gerrit Groenhof, Janne Ihalainen, Emina A. Stojković, Marius Schmidt & Sebastian Westenhoff. *Equally contribution. “The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography” Scientific Reports 6 35279 (2016) ::doi::10.1038/srep35279 Nils Lenngren*, Petra Edlund*, Heikki Takala*, Brigitte Stucki- Buchli, Ivan Peshev, Jessica Rumfeldt, Heikki Häkkänen, Sebastian Westenhoff, and Janne Ihalainen, “Coordination of the Biliverdin D-ring in Bacteriophytochromes”. Submitted (2018) *Equally contribution. Nicole C. Woitowich, Andrei S. Halavaty, Patricia Waltz, Christopher Kupitz, Joseph Varela, Gregory Tracy, Kevin D. Gallagher, Elin Claesson, Takanori Nakane, Suraj Pandey, Garrett Nelson, Rie Tanaka, Eriko Nango, Eiichi Mizohata, Shigeki Owada, Kensure Tono, Yasumasa Joti, Angela C. Nugent, Hardik Patel, Ayesha Mapara, James Hopkins, Phu Duong, Dorina Bizhga, Svetlana E. Kovaleva, Rachael St. Peter, Cynthia N. Hernandez,Wesley B. Ozarowski, Shatabdi Roy-Chowdhuri, Jay-How Yang, Petra Edlund, Heikki Takala, Janne Ihalainen, Jennifer Scales, Tyler Norwood, Ishwor Poudyal, Petra Fromme, John Spence, Keith Moffat, Sebastian Westenhoff, Marius Schmidt, & Emina A.Stojković.”Structural basis for light control of cell development revealed by crystal structures of a Myxobacterial phytochrome” Submitted (2017) Phytochromes Photosensors X-ray crystallography Serial femtosecond crystallography XFEL Structural biology Protein dynamics Structural Features of Bacteriophytochromes. Photoactivated Proteins studied by Serial Femtosecond Crystallography Text Doctoral thesis Doctor of Philosophy petra.edlund@gu.se University of Gothenburg. Faculty of Science Department of Chemistry and Molecular Biology ; Institutionen för kemi och molekylärbiologi Fredagen den 13:de april 2018, kl 9.00, Hörsal Åke Göransson, Medicinaregatan 11 2018-04-13 MNF open.access