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GUPEA >
Sahlgrenska Academy / Sahlgrenska akademin >
Institute of Neuroscience and Physiology / Inst för neurovetenskap och fysiologi >
Doctoral Theses / Doktorsavhandlingar >
Gastric Inhibitory Polypeptide in the brain
Please use this identifier to cite or link to this item:
http://hdl.handle.net/2077/19033
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| Title: | Gastric Inhibitory Polypeptide in the brain |
| Authors: | Nyberg, Jenny |
| Email: | jenny.nyberg@neuro.gu.se |
| Issue Date: | 2009-06-30 |
| University: | University of Gothenburg. Sahlgrenska Academy |
| Department: | Institute of Neuroscience and Physiology. Department of Clinical Neuroscience and Rehabilitation |
| Parts: | I. Ekaterina Perfilieva, Anette Risedal, Jenny Nyberg, Barbro J. Johansson, Peter S. Eriksson. Gender and strain influence on neurogenesis in dentate gyrus of young rats. J. Cereb. Blood. Flow. Metab. (2001), 21: 211-217.
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II. Jenny Nyberg, Michelle F. Anderson, Björn Meister, Ann-Marie Alborn, Anna-Karin Ström, Anke Brederlau, Ann-Christin Illerskog, Ola Nilsson, Timothy J. Kieffer, Max Albert Hietala, Anne Ricksten, Peter S. Eriksson. Glucose-Dependent Insulinotropic Polypeptide Is Expressed in Adult Hippocampus and Induces Progenitor Cell Proliferation. Journal of Neuroscience (2005), 25(7):1816 –1825.
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III. Jenny Nyberg, Calle Jacobsson, Michelle F. Anderson, Peter S. Eriksson. Immunohistochemical Distribution of Glucose-Dependent Insulinotropic Polypeptide in the Adult Rat Brain. J. Neurosc.i Research (2007), 85(10):2099-119.
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| Date for public defence: | 2009-01-16 |
| Public defence: | Fredagen den 16 januari 2009, kl. 13.00, Hörsal Arvid Carlsson, Academicum, Medicinaregatan 3, Göteborg |
| Examinationsnivå: | Doctor of Philosophy (Medicine) |
| Publication type: | Doctoral thesis |
| Keywords: | neural progenitor cells
neural stem cells
hippocampus
cell proliferation
GIP
neuropeptides
insulinotropic
gastrointestinal |
| Abstract: | The hippocampus is an area of active cell proliferation and neurogenesis within the adult
mammalian brain. Adult neurogenesis is of great importance for the brain, since it increases
neuroplasticity... more and the ability to respond to environmental stimuli. It also provides a possible
mechanism for the brain to replace lost cells that have died due to injury or disease. However, the molecular events controlling adult cell genesis in hippocampus remain largely unknown. One way of obtaining a deeper understanding of the mechanisms contributing to adult neurogenesis is to study the naturally occurring genetic variance in different inbred strains of rats while keeping the environment constant. In this thesis, we investigated differences in adult hippocampal neurogenesis between male and female rats of 2 different strains: Sprague-Dawley and Spontaneously Hypertensive. The aim of this project was to identify and investigate potential modulators of cell proliferation in the adult rat hippocampus. Combining BrdU, a marker of cell proliferation, with cell-specific markers, we found that Spontaneously Hypertensive rats had a higher rate of cell proliferation in the hippocampus than Sprague-Dawley rats. Moreover, male rats had more newborn cells than their female counterparts in both strains. To investigate this natural difference in proliferation rate and to identify potential regulators of cell genesis in the hippocampus, gene expression in the hippocampus was compared between the same groups of rats, using a cDNA array approach. Results revealed that hippocampal expression of the gene encoding glucose-dependent insulinotropic polypeptide (GIP) varied strongly in parallel with cell proliferation rates in the adult rat hippocampus. GIP is a polypeptide belonging to the secretinglucagon family of gastrointestinal regulatory polypeptides, and it was the only member of this peptide family that had not been described in the brain. To support our DNA results, we used immunohistochemistry and in situ hybridization to show that GIP mRNA and protein is expressed in the adult hippocampus and in cultured adult hippocampal progenitors. Furthermore, we found that the GIP receptor is also expressed by cultured adult hippocampal progenitors and throughout the granule cell layer of the dentate gyrus including progenitor cells in vivo. To confirm the proliferative capacity of GIP, we demonstrated that exogenously-delivered GIP induces proliferation of hippocampal progenitors in vivo as well as in vitro. Moreover, adult GIP receptor knockout mice exhibited a significantly lower number of newborn cells in the hippocampal dentate gyrus compared to wild-type mice. In order to investigate the localization of GIP-producing cells, we used immunohistochemistry on sections of the adult rat brain. We observed a widespread distribution of GIP-immunoreactive cells in the brain, with the highest level in the olfactory bulb, hippocampus and Purkinje cells of the cerebellum. This investigation demonstrates for the first time the presence of GIP in the brain and provides evidence for a regulatory function of GIP in progenitor cell proliferation. Although the importance of GIP in the different brain structures remains to be established, its widespread distribution suggests that it may play an important modulatory function in the brain. |
| ISBN: | 978-91-628-7675-3 |
| URI: | http://hdl.handle.net/2077/19033 |
| Appears in Collections: | Doctoral Theses from Sahlgrenska Academy
Doctoral Theses from University of Gothenburg / Doktorsavhandlingar från Göteborgs universitet
Doctoral Theses / Doktorsavhandlingar
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